Defra - Department for Environment, Food and Rural Affairs.

Science Search

Science and Research Projects

Return to Science Search homepage   Return to Search List

Method verification of the LOD associated with the Defra/FSA Study protocol for detection of horse DNA in food samples - FA0134

Food authenticity and food fraud are becoming increasingly problematic owing to pressures on food production and the current climate of financial constraint. The recent findings of horse DNA present in beef burgers sold in a UK supermarket chain has highlighted the need to provide support for rapid and reliable appraisal of the meat supply chain by developing standardised approaches for the detection and quantitation of different meat products.

Defra/FSA propose to conduct a UK wide study for identification of equine DNA in food samples using an FSA validated screening approach. This approach has an LOD associated with it that was based on approximate calculations. This limit of sensitivity needs to be robustly tested and qualified so that results from the study can be interpreted with confidence.
The aim of the proposed work is to use a statistically robust design to fully validate the LOD associated with the Defra/FSA study screening method, in terms of w/w (meat to meat) sample measurements. This will allow the results from the Defra/FSA Study on detection of equine DNA in food samples to be qualified and standardised in terms of a robust limit of detection.
Project Documents
• EVID4 - Final project report : Final report FA0134   (288k)
Time-Scale and Cost
From: 2013

To: 2013

Cost: £33,315
Contractor / Funded Organisations
LGC Limited
Agro Food Quality