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Improved detection of invasive aquatic plants- Cabomba caroliniana (fanwort), Myriophyllum heterophyllum (broadleaf watermilfoil) and Lagarosiphon major (curly waterweed) - BE0138

Description
The research project will assess the potential for DNA barcoding to detect aquatic invasive plant species Aquatic species are particularly difficult to identify, but DNA barcoding has shown promise for detecting aquatic animal species. The research will fund a literature review evidence that would support the development of DNA barcoding and other DNA detection methods for three important invasive species; Cabomba caroliniana (fanwort), Myriophyllum heterophyllum (broadleaf watermilfoil) and Lagarosiphon major (curly waterweed) and assess wider applicability for aquatic species
The first part of the project will include identifying whether DNA profiles for these species already exist and assessing tools that could be used to detect species in real time.
Objective
1) Carry out a literature review on the use of DNA methods (for identification and eDNA surveillance) for the three plant species of interest.
2) Identify and evaluate the suitability of the DNA markers available for the development of specific LAMP tests for the three invasive plant species: Cabomba caroliniana (fanwort), Myriophyllum heterophyllum (broadleaf watermilfoil) and Lagarosiphon major (curly waterweed).
3) Take forward the most useful markers (conserved with species and divergent between species) to the design of LAMP primers.
4) Perform specificity testing of the designed primer sets using material acquired commercially and through contacts within Defra.
Time-Scale and Cost
From: 2017

To: 2018

Cost: £62,100
Contractor / Funded Organisations
F E R A (FERA)
Keywords
Alien Species              
Biodiversity