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An investigation of the distinguishing features of C. jejuni strains that have host/disease implications - OZ0607

Description
Campylobacters are ubiquitous in the environment, but the consumption of contaminated food; particularly poultry meat is believed to be the main cause of human campylobacteriosis. Evidence suggests, however, that poultry may not be the sole source of human infection and other sources include domestic animals, water, milk and sub-populations of Campylobacter jejuni associated with a particular host or disease.

In order to identify the distinguishing features of sub-populations, which may act as markers of pathogenicity or host association, representatives of each group will be compared both phenotypically and at the genetic level. The role of these novel DNA sequences in pathogenicity will be investigated and the prevalence amongst veterinary isolates of Campylobacter jejuni investigated using microarrary technology.

Results will be used to add information to the currently available DNA microarrays to provide a more representative DNA chip, and also identify more representative isolates of the total population of Campylobacter jejuni which may be of use for future genome sequence projects.
Objective
1. To describe the population structure of campylobacters isolated from food-producing animals and humans.

2. To compare clonal group representatives both phenotypically and at the genetic level for the purpose of identifying markers of human pathogenicity and chicken/other food-producing animal colonisation.

3. To investigate the role of at least one selected marker in human pathogenicity and/or chicken colonisation.

4. Screen selected veterinary isolates for novel DNA/polypeptides identified in objectives 02.
Project Documents
• Final Report : An investigation of the distinguishing features of C.jejuni that have host/disease associations   (216k)
Time-Scale and Cost
From: 2001

To: 2004

Cost: £508,987
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Biotechnology              
Campylobacter              
GM Non-Food              
Plants and Animals              
Transmission              
Zoonoses              
Fields of Study
Animal Health