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Development of a peptide based ELISA for the measurement of anti-rabies antibody titres following vaccination - SE0416

Description
Currently there is no internationally approved enzyme linked immunoabsorbent assay (ELISA) which can be used to test companion animal sera for the presence of rabies virus specific antibodies in response to vaccination. the main obstacle to the development of such an assay is the production of an appropriate cost-effective antigen which does not require culture of live rabies virus. This project will approach this problem using synthetic peptides to act as a target antigen of an ELISA test. The objectives of this project arfe to identify the immunodominant epitopes on the rabies virus glycoprotein towhich dogs espond following vaccination with the Pasteur Virus (PV) strain. ONce identified, synthetic peptides for these linear sequences will be synthesised and tested for their suitability for use in a solid-phase ELISA test. Successful peptides will be assessed using panels of canine and feline sera obtained from submissions to the Pet Travel Scheme (PETS). The prototype assay will be validated against a large panel of post-vaccination sera and against the fluorescent antibody virus neutralisation (FAVN) test, the current Office International des Epizooties (OIE) gold standard. Further validation will include comparison to existing ELISA's currently undet development with AFFSA, Nancy, France and a commercial partner. The final outcome of this project will be a validated test for measuring rabies post-vaccination antibody responses in dogs ane cats.
Project Documents
• Final Report : Development of a peptide based ELISA for the measurement of anti-rabies antibody titres following vaccination   (2589k)
Time-Scale and Cost
From: 2001

To: 2004

Cost: £295,170
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Biotechnology              
Control              
Diagnosis              
Plants and Animals              
Rabies              
Zoonoses              
Fields of Study
Animal Health