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In vivo models to investigate the development of antibiotic resistance - OZ0502

The emergence of bacterial antibiotic resistance as a consequence of veterinaty antibiotic use is a major public health concern. The selective action of growth promoters and therapeutic antibiotics on organsims, especially foodborne pathogens in gut flora, have been particularly implicated in the spread of antibiotic resistance. The development of methods to predict and model the evolution of resistance in bacterial populations, prior to the licensing of new antibiotics, is a recommendation of the ACMSF. To accurately reproduce appropriate environmental conditions for such studies, in vivo models will be necessary. We have previously developed in vivo models to investigate the colonisation and/or pathogenicity of various foodborne bacterial pathogens in poultry. We propose to adapt these models to detect and investigate the kinetics and mechanisms of induction of resistance in defined bacterial pathogens by growth promoters alone or with relevent veterinary therapeutic antibiotics in the presence or absence of growth promoters. Models of Camylobacter jejune, Salmonella spp and E. coli colonisation are already avaliable for modification. An additional model of Enterococcus faecium colonisation will be developed. A mouse model will also be investigated in order to mimic the exposure of mammalian intestinal flora to antibiotic selection.
01: To establish oral models of colonisation witrh C. jejuni, S. enteritidis, S. typhimurium, E. coli or Enterococcus faecium in chickens with or without growth promoters.
02: To determine the effects of test antibiotics on antibiotic resistance of the colonising target organisms in the in vivo poultry model.
03: To deelop a murine model of porcine gut flora and use this to test for antibiotic resistance.
04: To determine the genetic basis of any observed antibiotic resistance in the target organisms.
05: To submit recommendations regarding the extension of the studies.
Project Documents
• Final Report : In vivo models to investigate the development of antibiotic resistance   (156k)
Time-Scale and Cost
From: 2000

To: 2004

Cost: £286,826
Contractor / Funded Organisations
Veterinary Laboratories Agency
Animal Diseases              
Animal Health              
Antimicrobial Resistance              
E.coli O157              
Plants and Animals              
Fields of Study
Animal Health