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Production, characterisation and utilisation of antibodies to ovine abnormal PrP for scrapie diagnosis and research - SE1761

Immunodetection methods for abnormal forms of PrP (PrPsc), play an important role in both diagnosis and research of the TSEs. Although in many areas these are the methods of choice, serious deficiencies in the availability of antibodies and the need to optimise and validate the new immunoreagents in existing protocols, continue to restrict their potential and use for the diagnosis and study of scrapie. This ROAME proposal presents the following course of study:
1. Production of antisera specific for ovine PrPsc and to selected PrP-peptides.
2. Assessment of commercially and collaboratively available antibodies
3. Assessment of available antibodies by immunohistochemistry (IHC); improvements to and optimisation of the IHC detection of ovine PrP in CNS and non-neural tissue.
4. Development of Immuno - based methods for tissues using PrPsc and PrP-peptide antisera.
MAFF are committed to setting up a scrapie surveillance programme. As with BSE, there are not even sufficient antibodies available to resource the programme as far as immunohistochemical and Western blot detections of PrP are concerned let alone undertake a development process for improved assays for ovine PrP for use in future scrapie surveillance and control.
Project Documents
• Final Report : Production, characterisation and utilization of antibodies to abnormal ovine PrP for the diagnosis of Scrapie   (1870k)
Time-Scale and Cost
From: 1999

To: 2002

Cost: £609,393
Contractor / Funded Organisations
Veterinary Laboratories Agency
Animal Health              
Plants and Animals              
Fields of Study
Animal Health