Defra - Department for Environment, Food and Rural Affairs.

Science Search

Science and Research Projects

Return to Science Search homepage   Return to Project List

Antibiotic Treatment of Commercial Broiler Flocks: Incidence and Mechanisms of Fluoroquinolone Resistance in Campylobact - OZ0501(3)

Description
Antibiotic resistance in zoonotic pathogens, including Campylobacter, isolated from man has been an increasing world wide issue, such that in October 1997 there was a World Health Organisation meeting to discuss the role that the use of antimicrobials in animals may contribute to the antibiotic resistance of these pathogens entering the food chain. Indeed concern was so great about fluoroquinolone resistance that a further WHO meeting in Geneva was held in June 1998, with experts from many parts of the world ranging from veterinary practitioners to farmers through to medical phsicians and scientists to discuss and provide data. With food safety a priority issue for the U.K government the 1998 report by the Working party of ACMSF investigating antibiotic resistance in food borne pathogens. In this proposal we address key issues that have been raised by these reports.The principal objectives of the proposed study are to investigate the incidence, and mechanisms of, fluoroquinolone resistance in Camplylobacter spp. from chickens and their immediate environment when poultry flocks are treated with the antibiotic. The project will determine whether fluoroqunilone-resistant mutants appear when approved treatment and husbandry regimes are followed, and will monitor the persistence of resistant bacteria once treatment has ceased. The new technique, denaturing (D) HPLC will be used as an accurate, high throughput method for determining the mechanism of fluoroquinolone and/or multi-drug resistance in Camplylobacter spp.. Analyses of the mechanism of resistance will provide fundamental data to DEFRA allowing informed decisions to be taken about use of fluoroquinolones in animals and consequent impact upon human health. The research will be relevant to DEFRA allowing informed decisions to be taken about use of fluoroquinolones in animals and consequent impact on human health. The research will be relevant to DEFRA policy as the rise in the prevalence of antibiotic reistant zoonotic pathogens could compromise the public health. There is a need to advise the poultry industry and the veterinary profession on treatment regimes, particularly when antibiotics are used on a large scale, and such advice is best based upon research using flokes of birds in commercial conditions. The results will be published in international peer reviewed and trade journals and disseminate information to the poultry industry by technology transfer.
Objective
01: To identify broiler flocks colonised with Campylobacter spp. and which will be treated with fluoroquinolone antibiotcs.
02: To set up systems with the collaborating poultry companies toe nable them to (1) alert the research groups when flocks will be treated with antibiotics and (ii) to keep an accurate record of antibiotic use.
03: To agree and use sensitive isolation and enumeration techniques for Campylobacter spp. and which will enable the detection of antibiotic-resistant organsisms.
04: To collect faecal and environmental samples from poultry flocks before, during and after antibiotic treatment.
05: To analyse collected organsism for resistance to fluoroquinolones and to determine the mechanisms of resistance.
06: Using D-HPLC to analyse isolates of Salmonella enterica and Campylobacter spp for resistance to fluoroquinolones and to determine the mechanisms of resistance.
07: To use the knowledge gained from 5 to develop molecular screening methods for specific resistance genotypes.
08: To type the Camplybacter isolated and determine the epidemiology of antibiotic-resistant isolates.
09: To use the above information to inform MAFF and the food industry of appropriate treatment and withdrawal regimes which will best protect the public health.
10: To produce a written report.
Time-Scale and Cost
From: 2000

To: 2003

Cost: £451,455
Contractor / Funded Organisations
University - Birmingham
Keywords
Animal Diseases              
Animal Health              
Antimicrobial Resistance              
Bacteria              
Biotechnology              
Campylobacter              
Plants and Animals              
Poultry              
Fields of Study
Animal Health