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To identify and characterise Campylobacters virulent for humans and derived from food producing animals - OZ0602

Description
Campylobacter jejuni and C.coli are the most common causes of acute bacterial enteritis in GB. Campylobacters are ubiquitous in the environment but contaminated foods - especially poultry meat, water and milk - are considered the major vehicles of infection. Preliminary evidence exists of variable virulence among strains from different sources. The presence of non-virulent strains, particularly in foods, could confound or bias epidemiological studies which primarily utilise data from total bacterial counts. Thus indicators of the relationships between strains and pathogenicity are urgently needed. Two approaches will be adopted: (1) to study the population biology of campylobacters in relation to host and disease; and (2) to identify the distribution of specific virulence-associated properties in campylobacters. For the first approach selected strains from veterinary, human and environmental sources will be phenotyped and genotyped by existing techniques like MEE and fla typing. Subtype and source relationships will be examined using population genetics methodology. For the second approach, the distribution of existing known virulence properties, such as toxin activity and invasion, will be determined. In addition, novel specific virulence factors will be activity sought using information from the genome sequence, mutagenesis and proteomics as part of the UK Genome Task Force.
Objective
Obj. No. Completed by date Description

01
31/03/2001
To analyse the population genetics of human, veterinary and environmental campylobacters.



02
31/03/2001
To identify and characterise novel virulence factors in C.jejuni as markers of pathogenicity.



03
31/03/2001
To determine the range of virulence properties of representative strains from human, veterinary and environmental sources using assays of invasion and toxin production.


04
31/03/2001
To provide estimates of the pathogenicity of campylobacter strains from veterinary sources.


Project Documents
• Executive Summary : To identify and characterise Campylobacters virulent for humans and derived from food producing animals   (23k)
• Final Report - Annex : To identify and characterise Campylobacters virulent for humans and derived from food producing animals   (118k)
Time-Scale and Cost
From: 1998

To: 2001

Cost: £627,980
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Biotechnology              
Campylobacter              
GM Non-Food              
Plants and Animals              
Transmission              
Zoonoses              
Fields of Study
Animal Health