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Molecular analysis of putative genetic factors affecting BSE susceptibility. - SE1744

Description
In addition to possible maternal transmission of BSE there are likely to be genetic factors that affect susceptibility to BSE. This project aims to investigate whether there are loci across the genome (not linked to PrP) which may be involved in susceptibility to BSE and, in conjunction with an EU project (studying flanking regions), will also assess whether PrP or linked loci play a role. A whole genome approach using microsatellite markers and AFLP techniques will identify such loci in bovine blood samples from large paternal half-sib families, including affected and non affected cattle. Samples from brain and serum from 84 cohort study calves will be studied to determine whether differences in frequency of polymorphism in the octapeptide PrP genotype exost between affected and unaffected individuals.
Objective
1. The main objective is to investigate whether there are loci other than PrP in the genome that play a role in infection or progression of BSE.
1.1 Large paternal half-sib families which include BSE affected vs unaffected progeny, will be used in linkage analysis using 200 microsatellite loci spanning the whole bovine genome.
1.2 DNA from BSE affected vs unaffected individuals will be pooled within and between families and used to search for differences between affected and unaffected indiciduals using Amplification Polymorphism Analysis (AFLP).

2. Through an EU contract a detailed study of the PrP gene, including non-coding sequence containing promoter and regulatory regions, to identify specific variations which may be associated with increased risk to disease.
2.1 Information obtained through the EU study will be used to establish whether variations in the PrP gene explain results from the cohort study.
Project Documents
• Final Report : Molecular analysis of putative genetic factors affecting BSE susceptibility   (68k)
Time-Scale and Cost
From: 1998

To: 2002

Cost: £477,154
Contractor / Funded Organisations
Roslin Institute, Edinburgh (BBSRC)
Keywords
Animal Health              
BSE              
Diagnosis              
Genetics              
Marker              
Plants and Animals              
TSEs              
Fields of Study
Animal Health