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BSE agent replication in bovine brain cell lines - SE1933

Bovine brain cell lines, after challenge with BSE agent, were passaged repeatedly to ensure that none of the inoculum or the original cells remained. Cells were then injected into RIII mice to determine whether the BSE infectious agent had replicated in the cultured cells. Mice with clinical symptoms of BSE are currently appearing after 500 days. However, analysis of passaged cells similar to those injected indicated that no proteinase K resistant PrPSc 27-30 was present. The main objective of this proposal is to determine whether the brains of the affected mice show accumulation of disease-specific PrPSc. This would be relevant to Research Requirement 4.1 “Does the presence of PrPSc correlate with infectivity?” in TSER and SU Research Requirements Document 2001/2002.
Project Documents
• Final Report : Web Final Summary   (31k)
Time-Scale and Cost
From: 1997

To: 2002

Cost: £211,317
Contractor / Funded Organisations
Moredun Research Institute
Animal Health              
Plants and Animals              
Fields of Study
Animal Health