Project Code: CE0312
Funded By Department for Environment, Food and Rural Affairs
Lead Research Centre
Central Science Laboratory,
Abstract of Research Proposal
To determine genetic variability in grain storage insects and mites and the adaptation of field strains to storage and pesticide practices. This will assist in the management of current pesticide resistance problems and, longer term, the development of strategies which will delay or prevent future possible control problems, including tolerance to modified atmospheres.
This work is directly relevant to policy objectives concerning the pursuit of cost-effective technologies which limit the use of pesticides to the minimum necessary for the effective control of pests. This is important to reducing the levels of both pesticide residues and pest in grain.
The results of this work will be used with results from other projects in the programme, to develop integrated management practices which contribute to maintaining and improving the quality of UK stored and traded grain and reduce pesticide use in store.
Summary of Objectives
1) To establish the genetic, biochemical or behavioural differences between strains of grain storage insects and mites, including common “field” strains.
2) To investigate whether there is significant genetic variability in tolerance of storage conditions, including inert atmospheres and cooled grain storage.
3) To investigate whether there is significant genetic variability in susceptibility to capture in traps and responses to pheromone lures.
4) To determine the extent of genetic, biochemical or behavioural differences in pests under conditions where control failures are occurring or where severe selection pressures may be imposed by physical/cultural methods or current pesticide use.
Summary of progress from 98/99 final report:
The major part of this first year of the project has been to collect samples of beetles from field locations, to establish cultures in the laboratory and to measure the baseline responses of those populations. All populations have been established in culture and the baseline responses fall within those expected for the species as a whole.
At the conception of the project it was anticipated that published methodology for DNA fingerprinting of insects could be applied directly to O. surinamensis. DNA fingerprinting of insects is less developed than for mammals and has not been used for beetles (Coleoptera) related to O. surinamensis. As a consequence it has been necessary to identify suitable microsatellite loci in the genome of O. surinamensis which has been delayed development of the full methodology.
Start date: 01/04/98
Completion date: 31/03/02