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The host specificity, pathogenesis and molecular classification of zoonotic protozoa isolated from livestock. - OZ0403

Description
This project aims to study differences between strains of zoonotic protozoa affecting humans and animals and to understand the role played by farm animals in the spread of human disease.
Whether pathogenic human strains of Cryptosporidium and Giardia are the same as those found in farm animals.
Whether farm animals can maintain pathogenic human strains, and would therefore be capable of functioning as a reservoir for human infection
To develop and validate molecular markers which will aid epidemiological studies and make interpretation of such work more objective.
These studies will contribute to answering the question of whether the majority of Cryptosporidium and Giardia infections are derived from animal contact or whether person-to-person transmission (either direct or indirect) is more significant.
Objective
01 31/03/1999 To type C. parvum isolates originating from cattle, sheep and humans in the UK, including samples obtained from major outbreaks if available during the lifetime of the project, and to set up a computerised database system to hold the DNA fingerprints which will also contain information pertaining to clinical history, pathogenicity of the isolate, host specificity, geographic area, etc. This will allow the identification of particular banding patterns which correlate with, for example, host specificity or pathogenesis.
02 31/03/2000 To discover whether human isolates will infect farm livestock and to assess the clinical symptoms and number of oocysts which are produced from such infections. The latter will allow an assessment of the potential environmental contamination for which domestic livestock may be responsible. The human isolates will be assessed to discover if any changes in DNA fingerprinting patterns occur, associated with passage in heterologous hosts.
03 31/03/2000 To type Giardia isolates originating from cattle, sheep and humans in the UK and to set up a computerised database system to hold the DNA fingerprints which will also contain information pertaining to clinical history, pathogenicity of the isolates, host specificity, geographic origin, etc. This will allow the identification of particular banding patterns which correlate with, for example, host specificity or pathogenesis.
04 31/03/2000 To discover whether human isolates of Giardia from the UK will infect farm livestock and to assess clinical symptoms of any infections and the number of oocysts which are produced. The latter will allow an assessment of the potential environmental contamination for which domestic livestock may be responsible. The human isolates will be assessed to discover if any changes in DNA fingerprinting patterns occur, associated with passage in heterologous hosts.
Project Documents
• Final Report : The host specificity, pathogenesis and molecular classification of zoonotic protozoa isolated from livestock.   (205k)
Time-Scale and Cost
From: 1997

To: 2000

Cost: £396,302
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Cryptosporidium              
Pathogenesis              
Plants and Animals              
Zoonoses              
Fields of Study
Animal Health