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Antioxidant nutrients and the cellular adaptation to oxidative stress in man - AN0438

Objective
1 and 2. Define the time course (objective 1) and magnitude of change (objective 2) of expression of the stress and adaptive responses following exhaustive exercise in non-supplemented subjects. Levels of HSP 27, 60, 70, 90 and 104 and the activities of the antioxidant enzymes catalase, superoxide dismutase, gluathione peroxidase and glutathione reductase will be examined in muscle samples obtained before and at set times following the exercise. The same proteins will also be examined in lymphocytes prior to, and following exposure to hydrogen peroxide in vitro. 3. Examine the effect of supplementation (for 8 weeks) with a high dose of vitamin E (400mg a-tocopherol acetate/day) on a) the stress response and antioxidant enzyme activities in muscle following exhaustive exercise, and b) the stress response and antioxidant activities of lymphocytes following exposure to H2O2 in vitro. 4. Examine the effect of prior supplementation (for 8 weeks) with a high dose of B-carotene (40mg B-carotene/ day a) the stress response and antioxidant enzyme activities in muscle following exhaustive exercise, and b) the stress response and antioxidant activities of lymphocytes following exposure to H2O2 in vitro. 5. Examine the effect of prior supplementation (for 8 weeks) with a high dose of vitamin C (1g vitamin C/day) on a) the stress response and antioxidant enzyme activities in muscle following exhaustive exercise and b) the stress response and antioxidant activities of lymphocytes following exposure to H2O2 in vitro.
Time-Scale and Cost
From: 1997

To: 1999

Cost: £115,881
Contractor / Funded Organisations
University - Liverpool
Keywords