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Dietary antioxidants in prevetion of tissue damage and changes in gene expression induced by UV free radicals - AN0424

Objective
1. Develop of methods to study expression of P53, C-fos , AP-1 and NF-B in human skin biopsies. 2. Determine the time course of changes in expression of P53, c-fos, Ap-1 and NF-B in human skin exposed to an acute challenge with UVB in vivo. 3. Determine the time curse of changes in indicators of free radical activity malonaldehyde, (oxidased glutathione, protein thiols and PUFA content) and antioxidants (vitamin E, carotenoids, vitamin C superoxide dismutase and catalase activity) in human skin exposed to an acute challenge with UVB. 4. Examine the effect of prior supplementation ( for 8 weeks) with a high dose of vitamin E (400mg a-tocopherol acetate/day) on changes in skin erythema, gene expression free radical activity and antioxidant content following acute challenge with UVB. 5. Examine the effect of prior supplementation (8 weeks) with a dose of b-carotene (40mg/day) on the above response to UVB. 6. Examine the effect of prior supplementation (for 8 weeks) with a high dose of vitamin C (1g/day) on the above reponse to UVB. 7. The most effective supplement observed in sections 4-6 will be studied in greater detail to determine the optional intake of the nutrient for prevention of UV-induced changes in skin ertheme and gene expression. 8. These studies will also allow us to determine the relationship between dietary intake of specific antioxidants and their incorporation in human skin.
Time-Scale and Cost
From: 1996

To: 1998

Cost: £99,552
Contractor / Funded Organisations
University - Liverpool
Keywords