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Dietary antioxidants in prevetion of tissue damage and changes in gene expression induced by UV free radicals - AN0424
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Objective
1. Develop of methods to study expression of P53, C-fos ,
AP-1 and NF-B in human skin
biopsies.
2. Determine the time course of changes in expression of
P53, c-fos, Ap-1 and NF-B in
human skin exposed to an acute challenge with UVB in vivo.
3. Determine the time curse of changes in indicators of free
radical activity malonaldehyde,
(oxidased glutathione, protein thiols and PUFA content) and
antioxidants (vitamin E,
carotenoids, vitamin C superoxide dismutase and catalase
activity) in human skin exposed
to an acute challenge with UVB.
4. Examine the effect of prior supplementation ( for 8
weeks) with a high dose of vitamin
E (400mg a-tocopherol acetate/day) on changes in skin
erythema, gene expression free
radical activity and antioxidant content following acute
challenge with UVB.
5. Examine the effect of prior supplementation (8 weeks)
with a dose of b-carotene
(40mg/day) on the above response to UVB.
6. Examine the effect of prior supplementation (for 8 weeks)
with a high dose of vitamin C
(1g/day) on the above reponse to UVB.
7. The most effective supplement observed in sections 4-6
will be studied in greater detail
to determine the optional intake of the nutrient for
prevention of UV-induced changes in
skin ertheme and gene expression.
8. These studies will also allow us to determine the
relationship between dietary intake of
specific antioxidants and their incorporation in human skin. |
Time-Scale and Cost
From:
1996
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To:
1998
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Cost: £99,552 |
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Contractor / Funded Organisations
University - Liverpool |
Keywords
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