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Potential for gene transfer between manipulated bacteria and the resident microflora of the human gut. - FS0207
1.To express lux and gfp marker genes in at least one strain
lactis and Enterococcus faecalis.
2.To introduce lux and/or gfp marker genes into the
chromosome of L. lactis.
3.To establish fermenter systems modelling the human hind
4.To establish and validate procedures for detecting lux and
sequences in DNA extracted from mixtures of human gut
added, marked strains, by PCR amplification.
5.To develop fluorimetric procedures for detecting and
marked lactic acid bacteria added to mixed human gut
6.To follow the fate of gfp-marked lactic acid bacteria and
gfp and lux gene
sequences introduced into fermenters that simulate
conditions in the human gut; to
screen for transfer to endogenous microflora.
7.To examine the stability of radiolabelled chromosomal DNA
Lactococcus lactis to gut conditions.
8.To study the influence of gut factors upon transfer of
chromosomal elements between Lactococcus strains and
endogenous lactic acid
bacteria from the gut.
9.To investigate acquisition of genetic elements by
introduced lactic acid
bacteria from endogenous microbes.
Time-Scale and Cost
Contractor / Funded Organisations
Rowett Research Institute