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The assessment and interpretation of diversity at the molecular and phenotypic levels in past and present varieties of Wheat, Barley and Oilseed Rape. - OC9424

Description
The assessment and interpretation of diversity at the molecular and phenotypic levels in past and present varieties of wheat, barley and oilseed rape.
Objective
The main objective is to obtain an assessment of genetic diversity in three major UK crops (wheat, barley and oilseed rape), using appropriate DNA profiling techniques, and to measure how diversity has changed over time. Molecular techniques to be used will include RFLP and RAPD in oilseed rape and the second generation techniques, AFLP and sequence-tagged microsatellites (STMS) in wheat and barley. Storage protein and morphological data will also be analysed as appropriate. Varieties of the three species will be chosen which represent the bulk of the UK crop at particular times. For wheat and barley the period of study will be 1934-1994 with varieties sampled at 10 yearly intervals, so that an assessment can be obtained of any changes in diversity patterns as a response to increased breeding effort during the past 70 years. In oilseed rape the development and turnover of varieties are relatively swift and material will be selected from a shorter total time period but sampled more frequently.
Phenotypic diversity will be established from existing morphological data for all three crops. Botanical characters contributing to phenotypoc diversity will be identifed. The relationship between genetic and phenotypic diversity will be assessed and related to known breeding pedigree.
Appropriate statistical methods will be developed to quantify and compare approaches to the assessment of total diversity and monitor any shifts in this diversity over time.
Time-Scale and Cost
From: 1995

To: 1998

Cost: £246,634
Contractor / Funded Organisations
John Innes Centre (BBSRC), National Institute of Agricultural Botany
Keywords
              
Fields of Study
Plant Varieties and Seeds