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The development of the Polymerase Chain Reaction for the quantitative detection of seed borne pathogens ....... - VS0109

Description
The development of the Polymerase Chain Reaction for the quantitative detection of seed borne pathogens and its evaluation for use in their control.
Objective
(i) Develop the technology of the simplex and multiplex polymerase chain reaction (PCR) in the identification and detection of seed-borne pathogens. This will provide the basis for the development of novel seed tests for organisms of importance to statutory certification schemes which will contribute to UK agriculture generally.
(ii) Evaluate the utility of these novel techniques in decision-making based on the results of seed tests.
(iii) Provide data on factors affecting the optimal conditions for multiples PCR using a model system.
(iv) Provide information on techniques to avoid PCR inhibition using a model system.
(v) Develop optimal techniques for quantifying results of simplex and multiplex PCR.
(vi) Develop suitable detection systems for PCR products using non-radioactive methods.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £238,500
Contractor / Funded Organisations
National Institute of Agricultural Botany
Keywords
              
Fields of Study
Plant Varieties and Seeds