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The role of diet in the modulation of human exposure to genotoxic agents as measured by DNA and protein adducts. - FS1716

Description
The purpose of this project is to investigate the use of biomarkers in the evaluation of the influence of dietary factors on human exposure to carcinogens. In this project, mass spectrometry, 32P-postlabelling and immunoassays will be used to assess 'background' DNA and protein damage (adducts) in humans eating standardised diets. The effect of intake of various specific dietary components eg ethyl alcohol, fats and proteins) will be assessed by measurements of the appropriate adducts. Subsequently, the effect of major dietary changes (eg high/low fibre)on background levels of adducts will be determined.
Objective
1. To establish background levels of DNA and Hb adducts in human volunteers on standardised diets. 2. To determine the effect of dietary change on these adduct levels. 3. To apply the methodology developed in this project to studies in humans where dietary intake could be manipulated, in order to identify dietary components which lower background levels of DNA and protein adducts.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £365,745
Contractor / Funded Organisations
Medical Research Council
Keywords