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A method for detecting Cryptosporidium parvum oocysts and Giardia duodenalis cysts in natural mineral waters. - FS1223

Description
Cryptosporidium parvum and Giardia duodenalis are protozoan parasited which occur frequently in a variety of UK water sources and are responsible for over eight waterbourn outbreaks of human disease. Legislation governing the quality of natural mineral waters for human consumption, require that parasited be absent. No standard method is available to demonstrate the presence of parasited in such waters. We propose to develop a method for the concentration , isolation and species identification of C. parvum oocysts and G. duodenalis cysts in large and small (bottled) volumes of natural mineral waters.
Objective
1. To evaluate the efficiency of both the SCA method and a membrane filtration method for the recovery of C. parvum oocysts and G. duodenalis cysts from large and small volumes of natural mineral waters. 2. To determine the effect of natural mineral waters upon the viability of C. parvum oocysts and G. duodenalis cysts over timew. 3. TO enhance the recovery efficiency of both the SCA and membrane filtration methods by developing an IMS method for the concentration of C. parvum oocyts and G. duodenalis cysts from natural mineral water concentrates. 4. To develop an effective IMS, PCR and gene probe-based species-specific identification method for C. parvum oocysts and G. duodenalis cysts , and to assess its reproducibility ,sensitivity and usefulness for species-specific detection of C. parvum oocysts and G. duodenalos cysts in natural mineral water concentrates.
Time-Scale and Cost
From: 1995

To: 1999

Cost: £255,053
Contractor / Funded Organisations
Scottish Parasite Diagnostic Laboratory
Keywords