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Pathogenic Yersinia enterocolitica; immunocontration and detection in foods. - FS1222
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Description
A novel virulence determinant of Yersinia enterocolitica,
namely the fimbrial antigen Myf, will be fully characterised
using immunological and molecular techniques. Expression of
the antigen in food products and culture media will be
determined and simple, rapid methods based on the
immunological and molecular detection of Myf developed to
detect pathogenic Y. enterocolitica strains in food. |
Objective
1. To produce immunological reagents:
a) rabbit polyclonal antibodies to Myf fimbriae.
b) Murine monoclonal antibodies to Myf fimbriae.
2. To design specific PCR assay to detect myfA gene sequence
in cultured strains of Y. enterocolitica
3. To detect Myf finbriae by pathogenic strains of Y.
enterocolitica.
4. To identify expression of Myf fimbriae by Y.
enterocolitica organisms present in food and environmental
samples :
a) detection by immunological methods
b) detection by molecular techniques
5. To develop methods for the separation, concentraion and
detection of pathogenic Y. enterocolitica strains from food
and environmental samples. Methods evaluated to include
immunomagnetic, chromatographic and membrane grid techniques
applied directly to samples or indirectly to enrichment
cultures. |
Time-Scale and Cost
From:
1994
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To:
1997
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Cost: £152,134 |
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Contractor / Funded Organisations
Veterinary Laboratories Agency |
Keywords
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