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Pathogenic Yersinia enterocolitica; immunocontration and detection in foods. - FS1222

Description
A novel virulence determinant of Yersinia enterocolitica, namely the fimbrial antigen Myf, will be fully characterised using immunological and molecular techniques. Expression of the antigen in food products and culture media will be determined and simple, rapid methods based on the immunological and molecular detection of Myf developed to detect pathogenic Y. enterocolitica strains in food.
Objective
1. To produce immunological reagents: a) rabbit polyclonal antibodies to Myf fimbriae. b) Murine monoclonal antibodies to Myf fimbriae. 2. To design specific PCR assay to detect myfA gene sequence in cultured strains of Y. enterocolitica 3. To detect Myf finbriae by pathogenic strains of Y. enterocolitica. 4. To identify expression of Myf fimbriae by Y. enterocolitica organisms present in food and environmental samples : a) detection by immunological methods b) detection by molecular techniques 5. To develop methods for the separation, concentraion and detection of pathogenic Y. enterocolitica strains from food and environmental samples. Methods evaluated to include immunomagnetic, chromatographic and membrane grid techniques applied directly to samples or indirectly to enrichment cultures.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £152,134
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords