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The effect of copper supplementation on measures of lipo- protein oxidation and DNA oxidant damage. - AN0511

Description
The purpose of the proposed project is to investigate the effect of oral supplementation of 12 healthy male and 12 healthy female volunteers (aged 20-40 years) with organically bound copper or inorganic copper on circulating cholesterol oxidation products in LDL ad HDL fractions; the susceptibility of LDL to in vitro iron-induced oxidizability ; and on lymphocyte DNA strand breakage and oxidised bases by the comet assay. The study design will be double-blinded repeated cross-over treatments with intervening placebo wash-out periods of 6 week duration. The following supplementations: copper sulphate; copper gluconate will be given in random sequence each for 6 week periods. Blood samples will be assessed for measures of oxidant damage or susceptibility to oxidation at the beginning of the study and at the end of each treatment and placebo period.
Objective
To provide information on whether different forms and levels of dietary copper supplements will affect indices of lipoprotein oxidation or DNA oxidant damage.
Time-Scale and Cost
From: 1995

To: 1998

Cost: £163,029
Contractor / Funded Organisations
University - Ulster
Keywords