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Experimental monitoring of GM bacteria using DNA probes. - RG0110

Objectives for this one year extension: i) To improve the detection limits for GM bacteria in field soils to within the range of 1-100 target genomes / g soil, providing a reliable and reproducible test that can be applied to any group of bacteria for which molecular probes can be generated. ii) To apply these methods to monitoring survival over 1y, of a genetically modified strain of Rhizobium which will b e released in the field for the first time in 1994. The development of the methodologies utilized the site of a previous release where numbers of GM rhizobia marked with Tn5 had stabilized at a low level. This current proposal is to follow a GM inoculant for the first year following its application, when, based on previous observations, viable cell numbers are predicted to drop from an initially high level of around 10(raised to the power of 5) cells per g, by 3-4 orders of magnitude.
Time-Scale and Cost
From: 1994

To: 1995

Cost: £40,951
Contractor / Funded Organisations
Rothamsted Research (BBSRC)
Fields of Study
Biotechnology and GMOs