Part of the assessment for the deliberate release of GMO's
involves a demonstration and quantification of the spread of
genes from a GMO and between natural bacterial species.
This work aims to examine GMO'S recovered from plants and
associated soil in a small scale release carried out
recently at Oxford (IVEM). The project aims to examine the
GMO's, recovered by the Oxford lab, first for bacteriophage
and then to determine the ability of the bacteriophage to
transduce the engineered genes. Thus we will:-
1. Examine GMO's, recovered over a period of time, for the
presence of bacteriophage which have come from the
associated soil microbial flora.
2. Test these bacteriophage for their ability to transfer
one of the recombinant genes to the parental
bacterium(Pseudomonas aureofasciens the parent of the GMO).
3. Examine the diversity of bacteriophage population
obtained. Various parameters will be examined, e.g . plaque
type, induction profile, yield, etc.
4. Preparation of report.