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Transfer of recombinant genes from a GMO by bacteriophage from soil - RG0109

Objective
Part of the assessment for the deliberate release of GMO's involves a demonstration and quantification of the spread of genes from a GMO and between natural bacterial species. This work aims to examine GMO'S recovered from plants and associated soil in a small scale release carried out recently at Oxford (IVEM). The project aims to examine the GMO's, recovered by the Oxford lab, first for bacteriophage and then to determine the ability of the bacteriophage to transduce the engineered genes. Thus we will:- 1. Examine GMO's, recovered over a period of time, for the presence of bacteriophage which have come from the associated soil microbial flora. 2. Test these bacteriophage for their ability to transfer one of the recombinant genes to the parental bacterium(Pseudomonas aureofasciens the parent of the GMO). 3. Examine the diversity of bacteriophage population obtained. Various parameters will be examined, e.g . plaque type, induction profile, yield, etc. 4. Preparation of report.
Time-Scale and Cost
From: 1994

To: 1994

Cost: £16,875
Contractor / Funded Organisations
University - Cardiff
Keywords
              
Fields of Study
Biotechnology and GMOs