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Risk assessment of genetically engineered avian probiotics. - RG0108

Objective
The hypothesis to be tested is that genetically engineered Lactobacillus and Enterococcus present no significant risk when used as avian probiotics. This hypothesis will be tested using genetically manipulated Lactobacillus and Enterococcus in which a beta-1, 4-glucanase is (a) present on a plasmid and maintained as an extrachromosomal element and (b) integrated into the chromosome. These constructs will be used to satisfy the following objectives(i) to evaluate whether the recombinant genes are transferred to other bacteria in the avian small intestine; (ii)to assess whether the impact of the genetically engineered organism on the microbial community of the small intestine differs significantly from that of the parent strain; (iii) to evaluate whether the genetically engineered organism persists and transfers its recombinant DNA in faeces and soil and (iv) to determine whether the introduction of beta-glucanase activity makes the engineered organisms more phytopathogenic.
Time-Scale and Cost
From: 1994

To: 1998

Cost: £121,098
Contractor / Funded Organisations
University - Newcastle
Keywords
              
Fields of Study
Biotechnology and GMOs