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Genetic techniques to detect E. coli 0157 - FS1211
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Description
Extremely sensitive techniques will be developed for the
detection of E. coli 0157, and other verocytotoxic E. coli i
foods of animal origin (meat, meat products, milk and dairy
products) using the polymerase chain reaction, PCR and
related techniques. Two approaches will be used, one to
detect specifically E.coli 0157 and the other to detect the
genes for verocytotoxin production. Having developed
suitable methods, assays will be performed on a) a
collection of E. coli strains to determine specificity and
sensitivity, b) spiked food samples and c) actual food
samples. |
Objective
1. To define and sequence DNA fragments which are unique to
E.coli 0157.
2. To develop a PCR specific for 0157.
3. To apply the 0157 specific detection systems using
methods and samples outlined in 4 and 5 below.
4. To apply non-radiolabelled probing systems such as the
combined PCR-ELISA to confirm specificity of amplification
product.
5. To enhance the sensitivity and specificity of existing
PCR techniques for the detection of VTEC.
6. To establish methods for the handling of food samples for
the optimal release of target DNA.
7. To evaluate the enhanced PCR for the detection of 0157
and other VTEC in food samples in both spiked and actual
purchased foods.
8. To consider at stage of the project the opportunity to
protect intellectual property rights by patent. |
Time-Scale and Cost
From:
1993
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To:
1996
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Cost: £66,345 |
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Contractor / Funded Organisations
Veterinary Laboratories Agency |
Keywords
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