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Measurement of human and animal biomarkers for aflatoxin exposure. - FS2016

It is proposed to use immunological and physioc-chemical methods to measure aflatoxin biomarkers in human tissues. These quantitative measures will be used to make comparisons with already published animal and human data relating tto biomarker level and aflatoxin exposure. Such studies will indicate whether or not the recently introduced aflatoxin regulations will have an impact on human aflatoxin biomarker level.
1. To establish sound, immunologically based methods with confirmation by physico-chemical procedures such as HPLC, to quantitate aflatoxin biomarkers viz aflatoxin B1- DNA adducts and aflatoxin lysine adducts, 2. To extend our preliminary abservations that aflatoxin-DNA adducts can be detected in a range of tissues in the UK population, 3. To examine on an individual basis the relationship between aflatoxin-DNA and aflatoxin lysine adducts, 4. To determine if a fall in the level of biomarkers will be seen after more stringent regulation of aflatoxin levels in foods, 5. To determine in experimental studies if endogenous formation of aflatoxins can occur.
Time-Scale and Cost
From: 1993

To: 1996

Cost: £208,540
Contractor / Funded Organisations
University of York, Jack Birch Unit