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Applying DNA-based analysis to operational monitoring: filling the barcode gap - ME6025

Description
The proposal here is a proof of concept study showing that small steps could bring the promise of DNA-based monitoring closer to reality for UK marine benthic habitats and help to deliver indicators for the Government's ambitious 25 year Environment Plan and the UK's international reporting. The use of DNA in assessing the biodiversity of sediments is expected to revolutionise the understanding of bottom-swelling marine communities. DNA approaches and advances have implications in terms of smarter, more expedient, comparatively cheaper and more easily applied monitoring activities which need to run alongside more traditional approaches in the first instance, to ensure calibration. DNA approaches may be the key to improving marine survey assessments in the future.
Objective
1) Preparation of a set of identified ethanol-preserved benthic invertebrate samples by traditional taxonomic methods.
2) Provide DNA barcodes for two markers for a prioritised set of the invertebrates (Obj 1). Metabarcoding results with be obtained and nalysed for an artificial community constructed from the identified invertebrates.
3) To obtain a comparison of morphological and molecular identifications, cross-testing both approaches, and use these to draw up the conclusions of the study.
Time-Scale and Cost
From: 2019

To: 2019

Cost: £30,909
Contractor / Funded Organisations
J N C C (JNCC - Joint Nature Conservation Committee) Support Committee
Keywords
Marine