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Validation of bioaerosol sampling approaches and devices – further work - WR0615

The overall aim of this project is to identify and characterise possible alternatives to sampling methods specified in the AfOR protocol. In particular:

• to compare the performance of CEN and impinger samplers.

• to strengthen the evidence base regarding the relationship between concentrations of particulate matter in samples and the concentrations of viable Aspergillus fumigatus spores and bacteria contained within the samples.
1. Test the collection efficiency under controlled conditions of three different types of impingers, specifically the SKC, AGI-30 and the VDI impinger, using known concentrations of Aspergillus fumigatus spores and a gram negative bacterium Pseudomonas fluorescens.

2. Test the stability of combined gelatine/polycarbonate filters used in the CEN device under a range of relative humidity conditions.

3. Test the survivability under controlled conditions of the more resistant Aspergillus fumigatus spores and the less resistant gram negative bacterium, Pseudomonas fluorescens, on gelatine/polycarbonate filters used with the CEN method over a time series. (The method has been designed for the longer term sampling (up to 24 hours) of resilient spores; as yet the survivability of bacteria on the gelatine filters over time has not been tested).

4. Assess the comparability between the CEN TS, an impinger method (impinger design will be based on the outcome of objective 1) and the Andersen (as an established reference sampler for monitoring ambient bioaerosol concentrations). The CEN TS is the European standard for monitoring moulds in air and therefore should be the method by which equivalence of any candidate bioaerosol sampling device is demonstrated. This project will determine the repeatability and equivalence of the impinger method to the CEN TS in the field.

5. In parallel with sampling for viable analysis in Objectives 4 and 5, measure the real time particulate concentration and size distribution.
Time-Scale and Cost
From: 2013

To: 2014

Cost: £60,279
Contractor / Funded Organisations
Health & Safety Laboratory, The Open University
Fields of Study
Waste Management