(a)Extend our investigations into the phylogeny,
distribution and abundance of transfer proficient plasmids
in bacteria indigenous to the phytosphere of crop plants.
(b) Investigate biotic (nutrient, plant species, plant age,
microbial population density) and abiotic (temperature, pH,
soil type, humidity) factors that influence conjugation in
situ using our rhizosphere gene transfer model (submitted
(c) Determine the extent to which natural transfer
proficient plasmids carry chromosomal mobilizing activity
(d) Investigate the frequency of plasmid mediated cma and
horizontal bacterial gene transfer in situ.