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A multiplex PCR to distinguish between M.bovis, M. bovis BCG and M. microti in clinical and environmental samples. - SE3236

Bovine tuberculosis is a chronic granulomatous disease mainly affecting lymph node and lung tissues of cattle. It is caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis (MTB) complex group of bacteria. At the genome level, M. bovis shares over 99% identity with Mycobacterium tuberculosis, the agent of human tuberculosis and other members of the MTB complex. Sequencing of key members of the MTB complex has shown differences due to deletions in the genomes which allow the development of methods for species identification. This proposal concerns the development of a multiplex PCR which will simultaneously detect and quantify three members of the MTB complex M. bovis, the vaccine strain M. bovis BCG, and M. microti, which mainly affects small mammals.
Project Documents
• Final Report : Final Report   (3560k)
Time-Scale and Cost
From: 2009

To: 2009

Cost: £31,540
Contractor / Funded Organisations
University - London - University College
Animal Diseases              
Animal Health              
Bovine Tuberculosis              
Disease Control              
Method Development              
Plants and Animals              
Fields of Study
Animal Health