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A new strategy to control tortricid pests in the orchard using baculovirus and pheromones (continuation of HH3108TTF) - HH3127STF

Four month continuation of project HH3108TTF to complete the processing and molecular biological analysis of stings and larvae removed from apples collected in orchard trials carried out on 12 ha of Bulmer’s apple orchards.

Apples have been collected at three assessment times, the last being on September 26th 2005. Processing and analysis of the samples is relatively time consuming.

Stings (entry damage caused by neonate larvae that pierce the apple surface and die as small larvae) and codling moth larvae from deep entry damage were collected at each assessment. The extracted stings will be fed to fifth instar larvae susceptible to codling moth granulovirus (CpGV) (LD50 only 10 OBs) and the larvae then checked for infection. It is possible to determine the strain of virus that caused the death of the larvae by differential PCR i.e. whether it was the applied strain in the autodisseminators. DNA will be extracted from the larvae and the strain of CpGV confirmed by PCR.
In addition, we have to sample and analyse from overwintering larvae as well as background samples.

Dye transfer experiments were conducted to determine the efficiency of the codling moth pheromone and a bisexual DA lure. The number of fluorescent contaminated insects has to be assessed by fluorescent microscopy.
1. Processing and analysis of sting damage and larvae from apples
collected from the field
2. Confirm efficacy of virus formulation
3. Determination and analysis of dye transfer experiment
4. Statistical analysis of field data completed
5. Final report written

final report published under project HH3108:
Time-Scale and Cost
From: 2005

To: 2006

Cost: £42,252
Contractor / Funded Organisations
Warwick - HRI, East Malling Research
Allocated - EMR              
Allocated - WHRI              
Biological Control              
Biotech-non GM              
Pest Control              
Sustainable Farming and Food              
Fields of Study