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A Rapid Sensitive Live Animal Blood Test for Sheep Scrapie - SE2006

The overall objective of the proposed project is the further development of Adlyfe’s Misfolded Protein Diagnostic (MPD) assay for TSE diseases in live animals. The MPD assay detects infectivity in a crude sample by identifying existing subpicomolar levels of infectious prion protein, PrPsc, through the use of specific, fluorescent peptide ligands (1). The labeled peptides undergo conformational change resulting in an amplified signal in a single step without protease pre-treatment, denaturants or washing. Specifically, we propose to validate the MPD assay protocol for sheep blood and conduct a time course study for endemic scrapie and controlled scrapie infection in Suffolk sheep in collaboration with the Moredun Research Institute. The time course study will allow us to pinpoint at what time the MPD assay is able to detect misfolded PrPsc in blood during the natural and induced course of scrapie disease. This evaluation will be performed in direct comparison with an accepted antibody-based method for PrPsc detection in lymph tissue. A rapid test for living animals is urgently needed for surveillance programs directed at TSEs in sheep, to allow fast and efficient detection of infectious prion proteins prior to the onset of clinical symptoms. It is envisioned that a successful outcome of the proposed study would help drive the MPD assay to be adopted and used for scrapie monitoring programs around the world.
Project Documents
• Final Report : A Rapid Sensitive Live Animal Blood Test for Sheep   (368k)
Time-Scale and Cost
From: 2005

To: 2008

Cost: £348,995
Contractor / Funded Organisations
Adlyfe Inc.
Animal Health              
Plants and Animals