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Physical factors affecting survival of ND and AI viruses in poultry products and the environment - SE4005

Description
The aim of this project is to generate up-to-date reliable data on the presence and survival of Newcastle disease (ND) and highly pathogenic avian influenza (HPAI; H5 and H7 viruses) in poultry products and excreta, and under different environmental conditions. Initially, studies will be carried out to determine the minimum infectious dose for ND and HPAI in three poultry species (chickens, turkeys and ducks), in both embryonated eggs and free-living forms. In addition for HPAI, the sensitivity of virus detection using embryonated eggs will be compared to real-time PCR, in order to establish if real-time PCR can be used to estimate levels of infectious virus. The effect of vaccination on virus shedding will also be investigated. Finally, work will be carried out to determine virus survival (i) in faeces of three poultry species at a range of temperatures (ii) in two sources of water and at a range of temperatures (iii) in poultry meat and egg products at a range of temperatures (iv) at different pHs and (v) following two feather decontamination processes. The information generated from the study will allow more reliable qualitative and quantitative risk assessments to be carried out, for trade and disease control purposes.
Objective
Objective 1: Define laboratory approaches and optimise methodology as required

Objective 2: Investigate minimum infectious dose for poultry

Objective 3: Define virus distribution and quantify levels in poultry products and excreta in vaccinated and unvaccinated hosts

Objective 4: Investigate the effects that physical factors have on virus survival
Project Documents
• Final Report : Physical factors affecting survival of ND and AI viruses in poultry products and the environment   (2977k)
Time-Scale and Cost
From: 2005

To: 2007

Cost: £197,580
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Diseases              
Animal Health              
Biosecurity              
Control              
Influenza              
Plants and Animals              
Fields of Study
Animal Health