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Investigation of polymorphisms associatedwith BSE in BARB cattle - SE0239

Description
The aim of this project is to establish whether there may be polymorphisms in the open reading frame (ORF) and promoter regions of the prion gene in born-after-the reinforced-ban (BARB) cattle associated with susceptibility to BSE disease.This project will use DNA sequencing to identify genetic variation in the prion protein loci of BARB cattle. 70 existing samples (blood, brain and homogenates) plus new cases may be added during the study. Control samples will be farm-, age-, sex- and breed-matched where possible.
The project is divided into 2 phases of work:
Phase 1: Sequencing the PrP ORF in BSE-affected and healthy control BARB cattle
Phase 2: Sequencing the promoter and known regulatory regions in BSE-affected and healthy control BARB cattle

A database of cattle sequence data will be established and maintained to optimise the representation of polymorphic sites. Finally, the data generated will be analysed for association between polymorphisms and disease, both for individual polymorphisms and predicted haplotypes. All the sequencing will be performed by QIAGEN, whereas Roslin Institute (John Williams) will provide expertise in statistical and haplotype analysis.
Future work on this topic could include: Sequencing the Doppel ORF in BSE-affected and healthy control BARB cattle and examining the frequency of BARB-associated polymorphisms in pre-96 BSE cattle.

Project Documents
• Final Report : Investigation of polymorphisms associated with BSE in BARB cattle   (138k)
• Technical Report : Investigation of polymorphisms associated with BSE in BARB cattle   (904k)
Time-Scale and Cost
From: 2004

To: 2006

Cost: £267,802
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
BSE              
Cattle              
Epidemiology              
Plants and Animals              
TSEs              
Fields of Study
Animal Health