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Development of immunohistochemical staining for Louping III virus in routinely processed tissues - SE0519

It is proposed to develop immunohistochemical staining techniques for the tick-borne Flavivirus Louping ill (LI) virus and the mosquito borne-flavivirus West Nile Fever (WNF) virus suitable for routinely processed animal tissues. Both WNFV and LIV can cause non-suppurative polioencephalitis typical of neurotropic viral infections.

WNFV can infect a wide range of mammalian and avian hosts, including man and horses (equine WNF encephalitis is a notifiable disease). Recently there have been outbreaks in temperate zones, most notably in the US, which were associated with human fatalities.

LIV mainly affects ruminants but also has a wide host range including grouse. Louping ill is the only currently recognised cause of neurotropic viral-type encephalitis in sheep in the UK. Outbreaks of Louping ill with unusual clinical presentations occurred in sheep and cattle in England and Wales in autumn 2001.

Development of an immunohistochemical test specific for Louping ill that is applicable to routinely processed tissues would allow rapid confirmation of cases of Louping ill that have unusual clinical presentations. An immunohistochemical test for WNF could be applied to diagnostic material either as a supplementary test to virus isolation and other tests or where the only available field material is fixed. The development of these two tests would provide a suite of immunohistochemical tests which would allow rapid resolution of suspect WNF cases encountered in the field.
1. Establish immunostaining for Louping ill virus
2. Optimise immunostaining techniques for West Nile Fever virus using monoclonal and rabbit polyclonal antibodies
Project Documents
• Final Report : Development of immunohistochemical staining for louping III virus routinely processed tissues   (38k)
Time-Scale and Cost
From: 2002

To: 2002

Cost: £5,000
Contractor / Funded Organisations
Veterinary Laboratories Agency
Animal Diseases              
Animal Health              
Plants and Animals              
Vector-Borne Viral Diseases              
Fields of Study
Animal Health