Defra - Department for Environment, Food and Rural Affairs.

Science Search

Science and Research Projects

Return to Science Search homepage   Return to Project List

The genotypic and phenotypic comparison of virulent Yersinia enterocolitica from humans and animals - OZ0405

Description
Yersinia enterocolitica is a Gram negative bacterium which can cause diarrhoea and related, more serious, diseases in humans. This infection is believed to be foodborne. Recent surveys indicate that 6% of cattle, 13% of sheep and 26% of pigs are colonised with this potential pathogen. However, the role of these veterinary strains in human disease is currently unknown. The aims of this project are to:
• To subtype veterinary strains of Y enterocolitica for comparison with human strains
• To use DNA arrays to investigate the genomic diversity of Y. enterocolitica strains from humans and animals
• To investigate the virulence potential of human and animal strains using a tissue culture model of invasion
• To evaluate two simple laboratory tests of putative virulence
The veterinary strains will be selected from available collections of Y.enterocolitica strains from national surveys of cattle, sheep and pigs at slaughter. Human strains will be selected from clinical cases reported to the PHLS. Other human strains will be obtained from the IID study with permission of the FSA. Once the phenotyping of these strains is complete selected strains will be genotyped using PFGE and AFLP. A database of this information will be generated. The data from the ongoing genome sequencing of Y. enterocolitica will then be used to construct DNA microarrays of all open reading frames. Up to 100 selected strains will be analysed using these arrays and areas of genetic variation identified. Data will be compared with the invasiveness of the strains in tissue culture assays as an indicator of a major virulence property. Finally two simple, rapid tests of potential virulence (arabitol utilisation and expression of Myf) will be investigated for possible routine laboratory use.

Objective
Objective O1. To subtype cattle sheep and pig strains of Y. enterocolitica for comparison with human strains

Objective 02. To use DNA microarrays to investigate the genomic diversity of Y.enterocolitica from humans and animals

Objective 03. To investigate the virulence potential of human and animal strains using a tissue culture model of invasion

Objective 04. To evaluate two simple laboratory tests of putative virulence of Y. enterocolitica in human and animal strains

Project Documents
• Final Report : The genotypic and phenotypic comparison of virulent Yersina enterocolitica from humans and animals   (1731k)
Time-Scale and Cost
From: 2002

To: 2005

Cost: £245,488
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Biotechnology              
GM Non-Food              
Pathogenesis              
Plants and Animals              
Yersinia              
Zoonoses              
Fields of Study
Animal Health