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The role of goats and pigs in the maintenance and transmission of VTEC including EHEC 0157:H7 - OZ0710

Description
This proposal is submitted in response to the call for research proposals for 2002 Animal Health & Welfare, under sections 3.3 Food Borne Zoonoses, subsection 3.3.2 Verotoxigenic E.coli, question R15

Escherichia coli O157:H7 is the cause of Haemolytic Uraemic Syndrome (HUS), Haemorrhagic Colitis (HC), Thrombotic Thrombocytopaenic Purpura and sporadic Infantile Diarrhoea in humans. These syndromes are potentially fatal in the young, elderly and immuno-compromised. Recent trace back and other epidemiological studies indicate that goats may be a significant source for the transmission and maintenance of E. coli O157:H7. Experimental infection of gnotobiotic and neonatal (12h of age) pigs with high doses of E. coli O157 is associated with neuronal damage (mediated by VT toxins) and diarrhoea (mediated by colonisation of the GI mucosa). However, nothing is known of the colonisation, persistence and excretion of E. coli O157:H7 in conventional goats and pigs. In order to assess the risk to human health arising from un-pasteurised goat’s milk and pig meat, it is essential that DEFRA is informed of the behaviour of E. coli O157:H7 in these hosts. Our laboratories have studied the pathobiology of E. coli O157:H7 in cattle and sheep and are well placed to undertake similar informative studies in goats and pigs. The aims of this proposal are (a) to study the pathogenicity in terms of colonisation, persistence and excretion of E. coli O157:H7 in goats and pigs, (b) to study potential transmission routes and (c) with respect to the goat study the mode of potential mammary infection.
Objective

No Due Description

01 31/03/04 To undertake porcine pathogenesis studies with EHEC O157 and other serotypes

02 31/03/04 To undertake porcine pathogenesis studies with EHEC O157 and other serotypes

03 31/03/04 To describe goat and pig EHEC O157isolates both genotypically and phenotypically,
including analysis in the surrogate persistence model

04 31/05/04 To complete all reports and submit papers relating to these studies

Project Documents
• Final Report : The role of goats and pigs in the maintenance and transmission of VTEC including EHEC 0157:H7   (313k)
Time-Scale and Cost
From: 2002

To: 2005

Cost: £399,546
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Biotechnology              
E.coli O157              
Plants and Animals              
Transmission              
Zoonoses              
Fields of Study
Animal Health