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The development of radioimmunoassays for the rainbow trout pituitary trophic hormones prolactin and growth hormone - FC0801

R&D Summary Objectives:- Develop methodology of hormone extraction, purification, antiserum generation and radiolabelling in order to measure hormone levels in trout blood plasma. Key customer purpose:- Develop an essential tool for the study of the role of hormones in growth and reproduction in fish.
a) Extraction and purification of prolactin and GH from rainbow trout pituitary glands. b) Production in rabbits of antisera against rainbow trout prolactin for detection of the hormone. c) Labelling of purified hormones with 125I to allow measurement of hormone binding in the assay. d) Using the purified prolactin and GH as standards, together with the labelled hormones and antisera, to produce prolactin binding curves as the basis of immunoassays.
Time-Scale and Cost
From: 1991

To: 1992

Cost: £38,405
Contractor / Funded Organisations
University - Cardiff
Fish Health              
Freshwater Fish Stocks              
Stock Conservation              
Fields of Study
Fish Health and Aquaculture