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Broad Spectrum Disease Resistance - HH0918SFV

The objective of this project is to:
- Investigate the potential of the SGT1 gene to provide a broad spectrum disease resistance against a wide range of bacterial, fungal, oomycete and viral pathogens in Arabidopsis and Brassica rapa.
- Identify the Brassica homolog of SGT1 and determine whether the gene functions in the same way in B.rapa as it does in Arabidopsis.
This project aims to determine whether the SGT1 gene would provide broad-spectrum disease resistance. the effect of SGT1 will be tested both in Arabidopsis and Brassica rapa. Brassica homologs og SGT1 will be identified and the function of the distinctive defence signalling gene will be investigated in Brassica. The information gained from this project will help breeders, pathologists and molecular biologists to understand and develop strategies to obtain sustainable disease control. This is an objective of DEFRA ROAME A HH109: Breeding Annual Crops

To isolate full-length cDNA of Arabidopsis and Brassica rapa SGT1 (AtSGT1 and BrSGT1)
To develop rapid and efficient transformation technology for Brassica
To generate Arabidopsis and Brassica plants over- and under-expressing SGT1
To generate Brassica plants under-expressing EDS1 to compare against SGT1 under-expressors.
To investigate the role of SGT1 in flowering in Arabidopsis.
To investigate expression level of SGT1 in Arabidopsis before and after pathogen attack.
To test Arabidopsis and B.rapa over and under-expressing SGT1 and EDS1 T2 lines with a wide range of pathogens including fungi, oomycetes, bacteria and viruses.

Nine-month supplemental project (2003-2004)
Molecular identification of avirulence determinants from an ascomycete pathogen of brassicas that reveal broad spectrum disease resistance in a non-host plant
Project Documents
• Final Report : Investigation of the potential for broad spectrum disease resistance in Brassica's using a novel defense signalling gene in Arabidopsis   (13704k)
Time-Scale and Cost
From: 2001

To: 2005

Cost: £448,295
Contractor / Funded Organisations
Horticulture Research International, Warwick - HRI
Allocated - WHRI              
Disease Control              
Organic Farming              
Pesticide use              
Sustainable Farming and Food              
Sustainable Production              
Fields of Study