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Smart plant technology for sensing crop nutritional status - AR0911
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Description
Abstract of Research Proposal
The project aims to identify plant genes whose expression is regulated by specific nutrient status, particularly by N and S. Genes will be evaluated for quantitative expression in relation to differential nutrient status in wheat. Promoters of these genes will be isolated and coupled to reporter genes and used in a transient expression assay system to evaluate responsiveness to nutritional status following re-introduction by bombardment. The promoters from this screening programme coupled to appropriate reporter genes (fluourescence, luminescence) will be suitable for introduction into transgenic crops or specific sentinel plants which are able to signal or communicate the nutritional status of the crop. Field-based detection would be through hand-held or tractor-based monitoring. This ‘smart plant’ technology will aid precision farming, avoiding uneconomic and potentially environmentally damaging excessive application of fertilisers.
Summary of Objectives
1. Identification and isolation of genes regulated by specific nutrient status using cDNA-AFLPs.
2. Examine robustness of expression and dose responsiveness with respect to applied fertiliser levels and quantified nutritional status.
3. Isolation of regulatory elements (promoters) responsible for nutrition regulation of gene expression, preparation of promoter-reporter constructs and use in transient assay analysis to assess effectiveness of reporting systems following re-introduction into plant tissues.
Funding Information
£210,000
Start date 1 December 2001
Completion date 30 November 2004
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Objective
1. Identification and isolation of genes regulated by specific nutrient status using cDNA-AFLPs
2. Examine robustness of expression and dose responsiveness with respect to applied fertiliser levels and quantified nutritional status
3. Isolation of regulatory elements (promoters) responsible for nutrition regulation of gene expression, preparation of promoter-reporter constructs and use in transient assay analysis to assess effectiveness of reporting systems following re-introduction into plant tissues
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Project Documents
• Final Report : Smart plant technology for sensing crop nutritional status
(5890k)
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Time-Scale and Cost
From:
2001
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To:
2004
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Cost: £209,608 |
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Contractor / Funded Organisations
Rothamsted Research (BBSRC) |
Keywords
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Fields of Study
Arable Crops |