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Reproducible Agrobacterium-mediated transformation systems for wheat and barley - AR1003

Although we have available routine transformation systems for wheat and barley based on particle bombardment, there are a number of reasons why routine Agrobacterium-mediated systems are desirable. Firstly, the transgenic plants produced using Agrobacterium-mediated approaches tend to have simpler integration patterns and lower copy numbers. Secondly, the Agrobacterium approach allows the possibility of removing marker genes by segregation in the progeny.

Therefore the main objective of this proposal is to develop routine and reproducible Agrobacterium-mediated transformation procedures for spring wheat and barley varieties Bobwhite and Golden Promise respectively.

The development of reproducible and transferable Agrobacterium-mediated techniques for the major UK cereal crops has been identified as a priority. It is expected that the results will show whether the Agrobacterium technique offers major advantages in terms of transformation efficiency, the quality of the transformation event, that is lower copy numbers and more precise integration, and in the removal of marker genes from transgenic lines.

The value of this work will be further enhanced by including lines developed by Agrobacterium-mediated techniques in field trials.
Objective 1:

To carry out large scale Agobacterium-mediated transformation experiments in wheat and barley that will lead to methodology that allows the reproducible production of transgenic plants.

Objective 2:

Subject to regulatory approval, confirm the value of the experimental transformation systems in field trials.
Project Documents
• Final Report : Reproducible Agrobacterium-mediated transformation systems for wheat and barley   (12k)
• Final Report - Annex : Reproducible Agrobacterium-mediated transformation systems for wheat and barley   (341k)
Time-Scale and Cost
From: 2000

To: 2001

Cost: £60,271
Contractor / Funded Organisations
John Innes Centre (BBSRC)
Arable Farming              
Crop Improvement              
Genetically modified food and crops              
GM Food              
Wheat Production              
Fields of Study
Arable Crops