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Classical Swine Fever: Viral proteins and virulence - SE0766

Description
The disease mechanisms involved in the clinical presentation of classical swine fever and the differing mortality seen in pigs infected with different strains of the virus are poorly understood. The main objective of this work is to understand something of the underlying virulence exhibited by classical swine fever by examining the way in which classical swine fever virus interacts with transcriptional, post-translational and immunological signalling events.
This will be achieved firstly by investigating the molecular mechanisms by which CSFV infection leads to an up- and down-regulation of cellular genes. This may allow for the prediction of the behaviour of different strains of CSF with varying virulence and the likely consequences of outbreaks. In addition, we will attempt to detect any physical interactions between cellular genes and proteins, and viral proteins, in order to elucidate the processes employed by the infecting virus to overcome the defence mechanisms that rely on cellular signalling to induce apoptosis, or programmed cell death.
The primary benefit of this work will be to aid MAFF in the formulation of strategies of control. This will assist in the development of efficient markets (MAFF objective 4) and the dissemination of knowledge gained will ultimately reduce suffering of animals (MAFF Objective 8). A reduction in disease losses caused by this virus will ensure reasonably priced pork and beef (MAFF Objective 10) which will directly assist the farming industry. This will also indirectly benefit the consumer by providing meat produced to a verifiable standard, at a competitive price.
Granting this project will help to maintain a nucleus of expertise within MAFF that provides up to date consultancy and diagnosis.
This research will provide valuable information in helping to predict the likely epidemiology of a particular virus strain causing an outbreak and could also lead to the development of therapies that could limit the viraemia and prevent in utero transmission, and hence the spread of virus. Such knowledge could also benefit the construction of safer, recombinant live vaccines.
Objective
1 01/09/02 - To identify differentially expressed host sequence tags following infection with CSFV isolates of differing virulence in order to identify candidate genes that may be involved in the differing virulence among strains of CSFV.


2 31/12/03 - To characterise candidate expressed sequence tags (ESTs) with respect to tissue localisation and temporal expression following infection.


3 31/12/03 - To indentify any viral proteins that may act as transcriptional regulators and toelucidate their role in inhibition of apoptosis.


4 27/02/04 – To identify any host proteins that may interact with viral proteins and to determine whether any inhibition of apoptosis may result.


5 30/09/04 – Determine if cell killing is dependent on the virulence of the infecting virus.


Project Documents
• Final Report : Classical swine fever: viral proteins and virulence   (421k)
Time-Scale and Cost
From: 2001

To: 2004

Cost: £354,310
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Diseases              
Animal Health              
Biotechnology              
Classical Swine Fever              
Plants and Animals              
Transmission              
Fields of Study
Animal Health