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Is the BSE strain phenotype stable on sub-passage in sheep? - SE1435

Description
BSE is experimentally transmissible to sheep with shortest incubation periods (so far) in animals of ARQ/ARQ PrP genotype. At IAH, we have already shown that infectivity,re-isolated from brain and spleen of such animals, retains the mouse transmission characteristics of primary BSE and have established the glycoform patterns of PrPSc in the sheep and mice.Several studies are underway at IAH,and elsewhere,to use this information to search for evidence of BSE in the UK national sheep flock.However,it is unlikely that many animals are alive at the moment that have been infected with primary BSE.

The aim of this proposal, therefore, is to put infectivity recovered from sheep clinically affected following experimental infection with primary BSE through a second round of sheep infection, and to establish whether, as a result of this second passage in sheep, there is any adaptation or difference in the subsequent mouse transmission properties and glycoform profiles as compared with the infectivity and PrPsc from the first round of infection.

The proposed study is relevant to MAFF policy on scrapie (development of alternative tests for scrapie - Scrapie ROAME A: A2.2, A3.3; specificity and sensitivity of alternative diagnostic tests - Scrapie ROAME A: A2.3) and on BSE (incidence of TSEs in other species-BSE ROAME A: A2. 2.1; possibility of subclinical infections 43SF ROAME A: A2.4.4; Diagnosis and Epidemiology - BSE ROAME A:A3.l and A3.2).

The results will indicate whether, if BSE has become established in UK sheep, its phenotype is stable and still recognisable by any of the currently used criteria.
Project Documents
• FRP - Final Report : Final Report SE1435   (199k)
• FRP - Final Report : Final Report SE1435 Figures 1 - 13   (127k)
• FRP - Final Report : Final Report SE1435 Tables 1-8   (227k)
Time-Scale and Cost
From: 2000

To: 2011

Cost: £3,808,879
Contractor / Funded Organisations
Institute for Animal Health (BBSRC), Roslin Institute, Edinburgh (BBSRC)
Keywords
Animal Health              
BSE              
Lesion Profile              
Plants and Animals              
Sheep              
Strain              
Transmission              
TSEs              
Fields of Study
Animal Health