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Molecular analysis of integrative transformants of the mushroom - HH0915SMU

The UK mushroom industry is the highest value crop of protected cultivation at £300m retail p.a. Growers have a continuing need for new and improved varieties (spawns) to remain competitive in the market place and to resist import peneteration. New spawns can potentially make a contribution to many of the problems faced by the industry. For example, they can help to mitigate the damaging effects of pest and diseases, to improve the post-harvest quality of mushrooms, and to meet market needs in respect of the developmental stage of the mushroom. For the mushroom the lack of an efficient transformation system for gene manipulation constrains directed strain improvement for the development of spawns with such characteristics. The objective of the research described here is to develop a system which removes this constraint.
Mushroom transformants will be generated using two different selectable marker genes, resistance to hygromycin and resistance to the fungicide carboxin. The fate, stability and expression of these genes will be determined for a defined number of transformants. The results from this project will enable the establishment of a transformation system which can be used in directed strain improvement. A utilisable transformation system will also enable the analysis of the biology of the mushroom at the gene level.
This under-pinning science, which conforms to MAFF guidelines for research using GM techniques, will expedite the production of novel spawns and the development of our understanding of mushroom biology. It will help meet the policy objective of enabling UK mushroom growers to maintain or extend their market share by improved competitiveness. The science will also impact on MAFF's wider mushroom R&D programme by providing a tool for understanding the role of key genes which impact on mushroom production e.g. genes involved in pathogenesis, crop agronomy, pre- and post-harvest quality, and substrate utilisation. Potential target genes identified in the wider MAFF programme can then be manipulated for strain improvement.
To develop (i) selectable markers, (ii) DNA delivery through agroinfection and (iii) an understanding of gene expression to the point where transformation can be used to deliver genes of biological or agronomic interest to a range of mushroom species.

Specifically, this will involve:

01. The production of at least 20 transgenic mushroom (A. bisporus) strains in which the location, expression and stability of the introduced transgene has been determined.

02. Testing the CBXR marker for the transformation of A. bitorquis, the oyster mushroom, Pleurotus ostreatus and the Japanese forest mushroom, Lentinus edodes.
Project Documents
• Final Report : Molecular analysis of integrative transformants of the mushroom   (8962k)
• Executive Summary : Molecular analysis of integrative transformants of the mushroom   (37k)
Time-Scale and Cost
From: 2000

To: 2003

Cost: £343,209
Contractor / Funded Organisations
Horticulture Research International
GM Food              
Fields of Study