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Detection of bovine prions from cattle with BSE using transgenic mice and conformation-dependent immunoassay (CDI) - SE1756

Description
Transgenic mice expressing bovine PrP are the most efficient animal bioassay sytem available. However an in vitro assay that could provide a similar level of sensitivity as well as being rapid and economical is desirable. This project aims to evaluate an incubation time assay for detecting BSE prions in tramsgenic mice overexpressing bovine PrP. the sensitivity of this model will be compared to that of detecting BSE and sheep scrapie prions in RIII mice. In addition a conformation-dependent immunoassay will be calibrated and evaluated for use with BSE samples as well as inoculated transgenics and assessed for sensitivity, specificity and positive and negative predictive value.
Objective
01. To evaulate an incubation time assay for detection of BSE [prions in transgenic mice
02. To simultaneously evaluate specificity and sensitivity of three different methods of BSE and sheep scrapie detection:
A. Bioassay in Tg mice overexpressing bovine PrP
B. Bioassay in wild-type RIII mice
C. Detection of BSE prions by conformation-dependent immunoassay (CDI).
Project Documents
• Final Report : Detecting of Bovine Prions from cattle with BSE using Transgenic Mice.   (292k)
Time-Scale and Cost
From: 2000

To: 2003

Cost: £1,723,693
Contractor / Funded Organisations
University - California
Keywords
Animal Health              
Bio-assay              
Biotechnology              
BSE              
CDI              
Diagnosis              
Plants and Animals              
Transgenic              
TSEs              
Fields of Study
Animal Health