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Fungal pathogen competitors of mushrooms. - HH1756SMU

Verticillium is the single largest threat of the pests and pathogens to mushroom production in the UK. None of the commercially grown strains has resistance and resistance to prochloraz pathogen, the only fungicide approved for control of the disease, has been detected.

The aim of this research is to determine the role of pathogenesis related enzymes produced by the pathogen Verticillium fungicola by selective inhibition and to identify hydrophobins produced by Verticillium and determine their role in the infecton of Agarican bisporus.

Genes coding for pathogen enzymes will be identified using heterologous probing. Transformation of Verticillium fungicola will be attempted using pAN7 (or alternative resistant markers) and Agroinfection and if successful, gene disruption of selected enzymes will be investigated.

This work relates to MAFF policy objectives of reducing usage which is considered environmentally desirable and would conform to consumer demand for reduced pesticide levels in food.
1. To establish transformation techniques for Verticillium fungicola.
2. To identify two key pathogensis related enzyme genes in Verticillium.
3. To disrupt pathogen enzyme activity to determine the effect on pathogenicity.
4. To identify hydrophobins within Verticillium fungicola.
5. To determine the role of hydrophobins in Agaricus and Verticillium in specificity.
Project Documents
• Final Report : Fungal pathogen competitors of mushrooms.   (706k)
Time-Scale and Cost
From: 1999

To: 2002

Cost: £237,563
Contractor / Funded Organisations
Horticulture Research International
Fields of Study