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Sono-chemical signal developmemt as a novel generic methods for the rapid, in situ and specific detection of - FS1245
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Description
low levels of food hazards at line |
Objective
1. Nanoparticulate enzyme
Enzyme preparations will be aggregated into small particles,
considering (bi-functional
cross-linking) physicochemical (precipitation), biochemical
(streptavidin) and physical
(dielectrophoretic, ultrasonic) methods. The quality of
particles (size, polydispersity) will
be monitored by dynamic light scattering (photon correlation
and diffusing wave
spectroscopies) and by assay of their relative activities.
2. Selection of sonochemical system
Sonochemical reaction systems will be reviewed and selected
according to their suitability
for signal generation in food matrices and their ability to
incorporate into particle systems
in a robust and stable manner.
3. Lipid coating of nanoparticulate enzyme
A procedure will be developed to coat the nanoparticulate
enzyme with a lipid-based layer
to block permeability. Sizes, poldisopersities,
concentrations, physical and biological
stability's will be similarly determined. Particular
attention will be devoted to attaining low
background activity and high stability under normal storage
and storage and treatment
conditions (pH, saly etc). Practical estimates will be made
of the number of particles
required to produce detectable vapour on activation by low
numbers of microorgansims.
4. Formulation of particulate sonochemical system
The sonschemical system selected will be produced as a
monodisperse sun-micron particle
suspension which can be incorporated into the responsive
particle system.
5. Specifically triggered vapour production
Our new peptide based biotin amplified system developed for
lipsomes will be
incorporated into the lipid coated nanoparticle system.
Coated manoparticles will be
modified with available antibodies for the pathogens so that
activity is triggered on binding
of the nanoparticle. Practical estimates will be made of
the triggering efficiency and
stability compared to the number and stability required for
useful measurement.
6. Incorporation of sonochemistry into responsive particle
Related methods will be developed to incorporate the
sonochemistry into the responsive
lipid coated system.
7. Demonstration of vapour and sonochemical signal detection
System to detect vapour and sound will be established and
used to compare signal
development on triggering.
8. Specific microbiological hazard detection
Known low number concentration of pathogen will be seeded
into various model media
and matrices and the vapour production and/or sound
generation monitored, compared to
controls and the count of organisms (where possible by
present methods ie>10/ml,
otherwise relying on known addition and back extrapolation
of growth curves). behaviour
will be considered in non-growing suspension, defined media,
complex growth media,
homogenised food plus growth and homogenised food.
9. Hazard detection on film for packaging
Colour chemistry for dosimetric of vapours on plastic film
will be developed and applied
to detecting specific vapour production on the presence flow
numbers of microorganisms
and compared to controls. PiezOptical detection of colour
development on PVDF film
will be evaluated.
10. Evaluation for at/on line hazard detection
The performance of vapour and sonochemical detection will be
optimised and evaluated to
enable comparison of their potential in microbiological
hazard detection, as a rapid
screening method in the laboratory, near real time
monitoring at or on line and for
revealing potential hazard in packaged food.
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Time-Scale and Cost
From:
1996
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To:
1999
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Cost: £346,035 |
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Contractor / Funded Organisations
University - Manchester |
Keywords
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