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Development of normal adult human colonic primary epithelial cell cultures and cell strains and their growth regulation - AN0312
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Objective
1.Reproduce the conditions for the short-term normal human
primary colonic
epithelial cultures previously developed. Assess the normal
primary cells
for markers of epithelial cell differentiation such as
keratins, CEA, E-
cadherin, mucins and domeing.
2.Test a range of growth factors and dietary factors for
their ability to act as
survival factors and increase the long term survival and
growth potential of
primary colonic colonic epithelial cultures.
3.Test extracellular matrix components for their ability to
act as survival
factors and increase the long-term survival and growth
potential of primary
colonic epithelial cultures.
4.Examine the effects of growth/dietary factors on the
expression of genes
implicated in growth control and cell survival in primary
cell cultures and
determine whether changes in gene expression are related to
the growth
response of the cells.
5.Determine the effects of dietary short-chain fatty acids,
calcium, and aspirin
on proliferation, differentation and apoptosis on primary
normal colonic
epithelial cells to determine their possible role in
maintaining normal tissue
homeostasis.
6.Using a range of techniques described above (1-4) develop
long term
cultures and isolate colonic epithelial cell strains from
normal adult colon
and passage these cells in vitro to obtain frozen stocks.
7.Determine the role of short-chain fatty acids, calcium and
aspirin in
regulating proliferation, differentation and apoptosis in
long-term epithelial
normal cell strains.
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Time-Scale and Cost
From:
1995
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To:
1997
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Cost: £78,315 |
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Contractor / Funded Organisations
University - Bristol |
Keywords
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