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Manipulation of lipid metabolism for production of fattyacids and polyketides for Industrial use and functional food. - AN0231
This project will be undertaken with 4 other European
Laboratories. In eukaryotic micro-organisms and plants,
acetyl-CoA ia a precursor for the synthesis of many
compounds including fatty acids and polyketides. The fatty
acids find increasing application in the food and chemical
industries. Some polyketides on the other hand are food
associated toxins, whereas others are widely usedas
antibiotics and pharmaceuticals. The fatty acid synthase (F
AS) and polyketide synthase (PKS) enzymes are remarkedly
similar in their function with with differences apparent
primarily in the detailed programming. Thees two enzymes
constitute a metabolic branch point between primary and sec
ondary metabolism. Fatty acids enter a second branch point
between membrane lipid biosynthesis (functional fats) and
fat accumulation 9storage fats) and this switch is
controlled by the acyl-CoA; diacylglycerol transferase
(DAGAT) enzyme. This proposal describes a research programm
e to investigate lipid and polyketide metabolism in yeasts,
filamentous fungi and oil-seed crop plants.
The project contains 6 main tasks. Each task includes a
number of specific scientific objectives which will take fo
ur years to complete.
1) To clone fatty acid synthase (FAS) and polyketide
synthase genes and study their relative activities.
2) To clone and study ATP: Citrate lyase and carnitive
acetyl transferase genes.
3) To study the production of short chain fatty acids using
heterologous FAS and thioesterase genes.
4) Study fatty acid desaturation using heterologous FAS and
5) Clone and regulate expression of gene encoding DAGAT.
6) Transfer lipid biosynthesis genes (T1-T5) to oil seed cr
Time-Scale and Cost
Contractor / Funded Organisations
IFR - Institute of Food Research (BBSRC)