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Dose response studies of dietary trans fatty acids on human lipoprotein metabolism - an investigation of mechanisms. - AN0230
1.Mechanistic studies in vitro: To identify which trans
fatty acids have
effects on cholesterol synthesis and LDL receptor expression
in human monocytes
and for those fatty acids having effects to define the dose
2.Trans fatty acid content of foods: To collect enough data
to give a
reasonably accurate estimate of the habitual trans fatty
acid intake of the subjects
recruited for our intervention study.
3.Intervention study: To examine the effects of a range of
intakes of trans
fatty acids, above, at and below current levels of intake in
the UK, on the
concentration of cholesterol and triglyceride in the various
and on the level of lipoprotein (a).
4.LDL expression in vivo:
(i)To examine the effects of a range of intakes of trans
fatty acids on LDL
(ii)To examine effects of dietary terms fatty acids (and
other fatty acids in
other projects) on the trans fatty acid content of the
membrane and LDL receptor expression in vivo.
(iii)To test the idea that LDL particles rich in trans fatty
acids are poor
substances for the LDL receptor.
5.Cholesterol synthesis in vivo: To examine the effects of
a range of intakes
of trans fatty acids on the rate of whole body cholesterol
synthesis in man in vivo.
Time-Scale and Cost
Contractor / Funded Organisations
IFR - Institute of Food Research (BBSRC)