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Dose response studies of dietary trans fatty acids on human lipoprotein metabolism - an investigation of mechanisms. - AN0230

Objective
1.Mechanistic studies in vitro: To identify which trans fatty acids have effects on cholesterol synthesis and LDL receptor expression in human monocytes and for those fatty acids having effects to define the dose response curve. 2.Trans fatty acid content of foods: To collect enough data to give a reasonably accurate estimate of the habitual trans fatty acid intake of the subjects recruited for our intervention study. 3.Intervention study: To examine the effects of a range of intakes of trans fatty acids, above, at and below current levels of intake in the UK, on the concentration of cholesterol and triglyceride in the various lipoprotein fractions and on the level of lipoprotein (a). 4.LDL expression in vivo: (i)To examine the effects of a range of intakes of trans fatty acids on LDL receptor expression. (ii)To examine effects of dietary terms fatty acids (and other fatty acids in other projects) on the trans fatty acid content of the endoplasmic reticulum membrane and LDL receptor expression in vivo. (iii)To test the idea that LDL particles rich in trans fatty acids are poor substances for the LDL receptor. 5.Cholesterol synthesis in vivo: To examine the effects of a range of intakes of trans fatty acids on the rate of whole body cholesterol synthesis in man in vivo.
Time-Scale and Cost
From: 1995

To: 1998

Cost: £473,498
Contractor / Funded Organisations
IFR - Institute of Food Research (BBSRC)
Keywords